Journal article
Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: Intracellular targeting to the amyloplast
MB Singh, T Hough, P Theerakulpisut, A Avjioglu, S Davies, PM Smith, P Taylor, RJ Simpson, LD Ward, J Mccluskey, R Puy, RB Knox
Proceedings of the National Academy of Sciences of the United States of America | NATL ACAD SCIENCES | Published : 1991
Abstract
We have identified a major allergenic protein from rye-grass pollen, tentatively designated Lol pIb of 31 kDa and with pI 9.0. A cDNA clone encoding Lol pIb has been isolated, sequenced, and characterized. Lol pIb is located mainly in the starch granules. This is a distinct allergen from Lol pI, which is located in the cytosol. Lol pIb is synthesized in pollen as a pre-allergen with a transit peptide targeting the allergen to amyloplasts. Epitope mapping of the fusion protein localized the IgE binding determinant in the C-terminal domain.