Journal article

Clinical value of sperm DNA damage should be assessed in motile sperm fraction rather than whole ejaculated sperm

De-Yi Liu, Ming-Li Liu

FERTILITY AND STERILITY | ELSEVIER SCIENCE INC | Published : 2013

Abstract

OBJECTIVE: To determine differences and frequency of excessive (≥50%) sperm DNA damage between ejaculated and motile sperm. DESIGN: Sperm DNA damage was assessed by acridine orange fluorescence and the results of ejaculated and motile sperm were compared. SETTING: Public and private clinical assisted reproduction centers. PATIENT(S): A total of 272 subfertile men were studied. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Semen analysis and sperm DNA damage. RESULT(S): Sperm DNA damage was negatively correlated with sperm motility and normal morphology. Overall, 39.7% (108 of 272) of semen samples had excessive sperm DNA damage. In contrast, only 15% (41 of 272) of motile sperm fractions h..

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