Journal article

EVIDENCE FOR A NOVEL PARATHYROID HORMONE-RELATED PROTEIN IN FETAL LAMB PARATHYROID-GLANDS AND SHEEP PLACENTA - COMPARISONS WITH A SIMILAR PROTEIN IMPLICATED IN HUMORAL HYPERCALCEMIA OF MALIGNANCY

CP RODDA, M KUBOTA, JA HEATH, PR EBELING, JM MOSELEY, AD CARE, IW CAPLE, TJ MARTIN

JOURNAL OF ENDOCRINOLOGY | J ENDOCRINOLOGY LTD | Published : 1988

Abstract

Parathyroid hormone (PTH)-like bioactivity, assayed as adenylate cyclase response in UMR 106-01 osteogenic sarcoma cells, was present in extracts of sheep fetal and maternal parathyroid glands and placenta. Preincubation of extracts with PTH(1-34) antiserum inhibited approximately 40% of the bioactivity in fetal parathyroid extracts, 50% in maternal parathyroid extracts, but only 10% of the bioactivity in the placental extract. Partial purification of placental extracts by chromatography yielded fractions containing PTH-like bioactivity which were similar in behaviour to that of PTH-related protein (PTHrP) from a human lung cancer cell line (BEN). An antiserum against synthetic PTHrP(1-16) p..

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University of Melbourne Researchers