Journal article

QUANTITATION OF TYROSINE-HYDROXYLASE AND NEUROPEPTIDE-Y IMMUNOREACTIVITY IN SINGLE-RAT SYMPATHETIC NEURONS - EFFECTS OF PREGANGLIONIC NERVE ACTIVITY

CJ GURUSINGHE, PJ HARRIS, DF ABBOTT, A MESSINA, C BELL

JOURNAL OF NEUROSCIENCE RESEARCH | WILEY-LISS | Published : 1991

Abstract

Using computerised densitometry to measure immunocytochemical reaction product in a model system, we established conditions that produced a linear relationship between the logarithm of antigen concentration and the measured intensity of staining. We then applied the densitometric technique to assess the changes in tyrosine hydroxylase (TH) and neuropeptide tyrosine (NPY) within sympathetic neurons of rat superior cervical ganglion following chronic decentralization and following reserpine treatment. One week after surgical or pharmacological decentralization, there was appreciable reduction of neuronal levels of both TH and NPY. However, there remained considerable variation in the immunorea..

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