Journal article

Using Drosophila melanogaster to validate metabolism-based insecticide resistance from insect pests

Phillip J Daborn, Christopher Lumb, Thomas WR Harrop, Alex Blasetti, Shivani Pasricha, Shai Morin, Sara N Mitchell, Martin J Donnelly, Pie Mueller, Philip Batterham

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2012

Abstract

Identifying molecular mechanisms of insecticide resistance is important for preserving insecticide efficacy, developing new insecticides and implementing insect control. The metabolic detoxification of insecticides is a widespread resistance mechanism. Enzymes with the potential to detoxify insecticides are commonly encoded by members of the large cytochrome P450, glutathione S-transferase and carboxylesterase gene families, all rapidly evolving in insects. Here, we demonstrate that the model insect Drosophila melanogaster is useful for functionally validating the role of metabolic enzymes in conferring metabolism-based insecticide resistance. Alleles of three well-characterized genes from d..

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