The design and proof of concept for a CD8( ) T cell-based vaccine inducing cross-subtype protection against influenza A virus
Amabel CL Tan, Georgia Deliyannis, Mandvi Bharadwaj, Lorena E Brown, Weiguang Zeng, David C Jackson
IMMUNOLOGY AND CELL BIOLOGY | NATURE PUBLISHING GROUP | Published : 2013
In this study, we examined the reactivity of human peripheral blood mononuclear cells to a panel of influenza A virus (IAV) CD8(+) T-cell epitopes that are recognised by the major human leukocyte antigen (HLA) groups represented in the human population. We examined the level of recognition in a sample of the human population and the potential coverage that could be achieved if these were incorporated into a T-cell epitope-based vaccine. We then designed a candidate influenza vaccine that incorporated three of the examined HLA-A2-restricted influenza epitopes into Pam2Cys-based lipopeptides. These lipopeptides do not require the addition of an adjuvant and can be delivered directly to the res..View full abstract
This work was funded by grants provided by the National Health and Medical Research Council of Australia. We thank Dr F Lemonnier and the Institute Pasteur for permission to use the HHD mice and Dr A Suhrbier (Queensland Institute for Medical Research) for supplying the breeding stock. Many thanks to the volunteers for kindly providing the blood samples used in this study and to Associate Professor Joseph Torresi (The University of Melbourne) for his assistance in blood sample collection.