Journal article

Differential Proteomic Analysis of a Polymicrobial Biofilm

Zamirah Zainal-Abidin, Paul D Veith, Stuart G Dashper, Ying Zhu, Deanne V Catmull, Yu-Yen Chen, Deasy C Heryanto, Dina Chen, James S Pyke, Kheng Tan, Helen L Mitchell, Eric C Reynolds

JOURNAL OF PROTEOME RESEARCH | AMER CHEMICAL SOC | Published : 2012

Abstract

Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia exist in a polymicrobial biofilm associated with chronic periodontitis. The aim of this study was to culture these three species as a polymicrobial biofilm and to determine proteins important for bacterial interactions. In a flow cell all three species attached and grew as a biofilm; however, after 90 h of culture P. gingivalis and T. denticola were closely associated and dominated the polymicrobial biofilm. For comparison, planktonic cultures of P. gingivalis and T. denticola were grown separately in continuous culture. Whole cell lysates were subjected to SDS-PAGE, followed by in-gel proteolytic H₂¹⁶O/H₂¹⁸O labeling. F..

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