The treatment of established intracranial tumors by in situ retroviral IFN-γ transfer

Abstract

Current treatments for malignant gliomas are still largely ineffective in significantly improving prognosis. We have investigated the efficacy of treating established rat C6 glioma by in situ retroviral delivery of IFN-γ cDNA. Ecotropic retrovirus packaging cells were transfected with a retroviral vector containing the mouse IFN-γ gene. The IFN-γ packaging cells were stereotactically implanted into established intracranial C6 glioma in immunocompetent Wistar rats, resulting in the eradication of these tumors. All IFN-γ treated rats survived to 92 days after C6 implantation (an arbitrary end point) compared with 14 days for controls. Analysis of these treated brains showed that the establishe..