High-Throughput PCR Assays To Monitor Wolbachia Infection in the Dengue Mosquito (Aedes aegypti) and Drosophila simulans
Siu F Lee, Vanessa L White, Andrew R Weeks, Ary A Hoffmann, Nancy M Endersby
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | AMER SOC MICROBIOLOGY | Published : 2012
We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening.
The study was funded by a grant from the Foundation for the National Institutes of Health through the Grand Challenges in Global Health Initiative as well as the CSIRO Cluster Collaboration Fund "Urbanism, Climate Change and Health" and fellowships from the Australian Research Council.