Chimeric relaxin peptides highlight the role of the A-chain in the function of H2 relaxin
Mohammed Akhter Hossain, John D Wade, Ross AD Bathgate
Peptides | ELSEVIER SCIENCE INC | Published : 2012
Human gene-2 (H2) relaxin is a member of the insulin-relaxin peptide superfamily. Because of the potential clinical applications of H2 relaxin, there is a need for novel analogs that have improved biological activity and receptor specificity. In this respect, we have chemically assembled chimeric peptides consisting of the B-chain of H2 relaxin in combination with A-chains from other insulin/relaxin family members. The peptides were prepared using solid phase peptide synthesis together with regioselective disulfide bond formation and characterized by RP-HPLC, MALDI-TOF MS and amino acid analysis. Their in vitro activity was assessed in RXFP1 or RXFP2 expressing cells. Replacement of the H2 r..View full abstract
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Awarded by NHMRC (Australia)
This research was funded by NHMRC (Australia) project grants 350284, 508995 to JDW and RADB. We are grateful to Tania Ferraro & Sharon Layfield for assistance with activity assays and to Feng Lin for amino acid analysis. During these studies, MAH was the recipient of a Reid Trust Fellowship and a Florey Trust Foundation Fellowship. RADB is an NHMRC Senior Research Fellow. JDW is an NHMRC Principal Research Fellow. Studies at the FNI were supported by the Victorian Government's Operational Infrastructure Support Program.