Development and Optimization of MicroRNA against Relaxin-3
Gabrielle E Callander, Walter G Thomas, Ross AD Bathgate, GD BryantGreenwood (ed.), CA Bagnell (ed.), RAD Bathgate (ed.)
RELAXIN AND RELATED PEPTIDES: FIFTH INTERNATIONAL CONFERENCE | WILEY-BLACKWELL | Published : 2009
Recent in vivo studies suggest a role for relaxin-3 in feeding and stress. To further elucidate the function of relaxin-3 in the central nervous system, we have employed a complementary approach, based on RNA interference, to modulate relaxin-3 expression. We have designed, constructed, and characterized three microRNAs (miRNAs) targeting different regions of the relaxin-3 transcript. These were tested to determine the amount of miRNA required to achieve the greatest knockdown and for their ability to reduce the expression of relaxin-3 in transfected HEK293Tcells. All miRNA constructs significantly reduced relaxin-3-induced cAMP responses; however, miR499 was most effective. This should be a..View full abstract
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Awarded by National Health and Medical Research Council of Australia
Dr. Ross Bathgate and Prof. Walter Thomas are recipients of fellowships from the National Health and Medical Research Council of Australia (509011 and 317814). Gabrielle Callander is a recipient of a Melbourne Research Scholarship. This work was supported by project grants 300012 and 454375 (RADB) and 418921 (WGT) from the National Health and Medical Research Council of Australia.