Journal article

PRODUCTION AND IN-VIVO USE OF RECOMBINANT OVINE IL-1-BETA AS AN IMMUNOLOGICAL ADJUVANT

AE ANDREWS, SA LOFTHOUSE, VM BOWLES, MR BRANDON, AD NASH

VACCINE | ELSEVIER SCI LTD | Published : 1994

Abstract

To determine the potential of ovine interleukin 1 (IL-1) as a vaccine adjuvant in sheep, we have expressed and purified recombinant ovine IL-1 beta (rovIL-1 beta) from bacterial cultures using a modified form of the ovine IL-1 beta cDNA. Adjuvant trials using the model protein avidin demonstrated that rovIL-1 beta when administered in association with a compound providing a slow-release mechanism, resulted in significant enhancement of specific serum antibody levels in both mice and sheep. In a dose-response experiment in sheep, intradermal immunization with avidin plus either 10 or 100 micrograms of rovIL-1 beta in aluminium hydroxide resulted in antibody levels four- to eightfold higher th..

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University of Melbourne Researchers