Involvement of the mannose receptor in infection of macrophages by influenza virus

Abstract

Influenza viruses A/PR/8/34 (PR8; HIN1), A/Aichi/68 X-31 (HKx31; H3N2), and A/Beijing/89 X-109 (BJxl09; H3N2) show marked differences in their ability to infect murine macrophages, including resident alveolar and peritoneal macrophages as well as the macrophage-derived cell line J774. The hierarchy in infectivity of the viruses (PR8 < HKx31 < BJxl09) resembles that of their reactivity with mannose-binding lectins of the collectin family. Since the macrophage mannose receptor recognizes the same spectrum of monosaccharides as the collectins do, we investigated the possible involvement of this receptor in infection of macrophages by influenza virus. In competitive binding studies, the binding ..