Journal article

MODULATION OF GLUCOSE-TRANSPORT BY PARATHYROID-HORMONE AND INSULIN IN UMR-106-01, A CLONAL RAT OSTEOGENIC-SARCOMA CELL-LINE

DM THOMAS, SD ROGERS, MW SLEEMAN, GM PASQUINI, FR BRINGHURST, KW NG, JD ZAJAC, JD BEST

JOURNAL OF MOLECULAR ENDOCRINOLOGY | BIOSCIENTIFICA LTD | Published : 1995

Abstract

This study characterizes the actions of insulin and parathyroid hormone (PTH) on the glucose transport system in the rat osteogenic sarcoma cell line UMR 106-01, which expresses a number of features of the osteoblast phenotype. Using [1,2-3H]2-deoxyglucose (2-DOG) as a label, UMR 106-01 cells were shown to possess a glucose transport system which was enhanced by insulin. In contrast, PTH influenced glucose transport in a biphasic manner with a stimulatory effect at 1 h and a more potent inhibitory effect at 16 h on basal and insulin-stimulated 2-DOG transport. To explore the mechanism of PTH action, a direct agonist of cAMP-dependent protein kinase (PKA) was tested. 8-Bromo-cAMP had no acute..

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