Journal article

NKX2-5eGFPw hESCs for isolation of human cardiac progenitors and cardiomyocytes

David A Elliott, Stefan R Braam, Katerina Koutsis, Elizabeth S Ng, Robert Jenny, Ebba L Lagerqvist, Christine Biben, Tanya Hatzistavrou, Claire E Hirst, Qing C Yu, Rhys JP Skelton, Dorien Ward-van Oostwaard, Sue Mei Lim, Ouda Khammy, Xueling Li, Susan M Hawes, Richard P Davis, Adam L Goulburn, Robert Passier, Owen WJ Prall Show all

NATURE METHODS | NATURE PUBLISHING GROUP | Published : 2011

Abstract

NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.

Grants

Awarded by National Health and Medical Research Council of Australia


Awarded by National Heart Foundation (Australia)


Funding Acknowledgements

This work was funded by the Australian Stem Cell Centre (to E.G.S., A.G.E., D.A.E., D.M.K., J.M.H. and C.W.P.), the National Health and Medical Research Council of Australia (D.A.E., grant 606586; O.W.J.P., grant 573707), National Heart Foundation (Australia) (E.G.S. and A.G.E., grant G 08M 3711; O.W.J.P., Career Development Award grant CR 08S 3958), the Netherlands Organization for Scientific Research and the Netherlands Institute of Regenerative Medicine (S.R.B. and C.L.M.), the Victorian State Government Operational Infrastructure Support and the National Health and Medical Research Council of Australia's Independent Research Institutes Infrastructure Support Scheme (O.W.J.P. and C.B.). R.P.D. is funded by a Rubicon fellowship from the Netherlands Organization for Scientific Research and the Marie Curie Co-fund Action. E.G.S. and A.G.E. receive Senior Research Fellowships and D.M.K. receives a Principal Research Fellowship from the National Health and Medical Research Council of Australia.