Journal article

Efficient cre-mediated deletion in cardiac progenitor cells conferred by a 3 ' UTR-ires-Cre allele of the homeobox gene Nkx2-5

EG Stanley, C Biben, A Elefanty, L Barnett, F Koentgen, L Robb, RP Harvey

INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY | UNIV BASQUE COUNTRY UPV-EHU PRESS | Published : 2002

Abstract

Conditional gene targeting and transgenic strategies utilizing Cre recombinase have been successfully applied to the analysis of development in mouse embryos. To create a conditional system applicable to heart progenitor cells, a Cre recombinase gene linked at its 5' end to an internal ribosome entry site (IRES) was inserted into the 3' untranslated region of the cardiac homeobox gene Nkx2-5 using gene targeting. Nkx2-5IRESCre mice were fully viable as homozygotes. We evaluated the efficacy of Cre-mediated deletion by crossing Nkx2-5IRESCre mice with the Cre-dependent R26R and Z/AP reporter strains. Efficient deletion was observed in the cardiac crescent and heart tube in both strains. Howev..

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