Chamber formation and morphogenesis in the developing mammalian heart.
VM Christoffels, PE Habets, D Franco, M Campione, F de Jong, WH Lamers, ZZ Bao, S Palmer, C Biben, RP Harvey, AF Moorman
Dev Biol | Published : 2000
In this study we challenge the generally accepted view that cardiac chambers form from an array of segmental primordia arranged along the anteroposterior axis of the linear and looping heart tube. We traced the spatial pattern of expression of genes encoding atrial natriuretic factor, sarcoplasmic reticulum calcium ATPase, Chisel, Irx5, Irx4, myosin light chain 2v, and beta-myosin heavy chain and related these to morphogenesis. Based on the patterns we propose a two-step model for chamber formation in the embryonic heart. First, a linear heart forms, which is composed of "primary" myocardium that nonetheless shows polarity in phenotype and gene expression along its anteroposterior and dorsov..View full abstract