Effects of acute and chronic counterregulatory hormone infusions on glucose tolerance and insulin sensitivity in diabetic dogs

Abstract

The effects of elevated EPI and CORT levels on KG, SI, and SG were studied in dogs with alloxan-induced diabetes. Conscious dogs received SAL, EPI 20 ng kg-1 min-1 for 30 min (short EPI) or 72 h (long EPI), or CORT 200 μg kg-1 min-1 for 60 min (short CORT) or 72 h (long CORT) before assessment of glucose metabolism by rapid sampling for glucose and insulin levels after 300 mg/kg i.v. glucose and exogenous insulin infusion designed to simulate the normal secretory pattern. With EPI infusion, KG fell acutely from 2.9 ± 0.4 to 2.0 ± 0.2%/min (SAL vs. short EPI, P < 0.05), but rose to 3.4 ± 0.4%/min during long EPI. Minimal-model analysis of the glucose response with the insulin data as input sh..