Journal article

Determination of angiotensin I-converting enzyme activity in equine blood: lack of agreement between methods of analysis

Maria Fernanda de M Costa, Adriana K Carmona, Marcio FM Alves, Timothy M Ryan, Helen M Davies, Garry A Anderson, Ron F Slocombe

Journal of Veterinary Science | KOREAN SOC VETERINARY SCIENCE | Published : 2011

Abstract

Angiotensin-I converting enzyme (ACE) is a key regulator of blood pressure, electrolytes and fluid homeostasis through conversion of angiotensin I into angiotensin II. Recently, a genetic polymorphism of the ACE gene, which accounts for 47% of the variation of ACE activity in blood, has been advocated as a biomarker of athletic aptitude. Different methods of analysis and determination of ACE activity in plasma have been used in human and equine research without a consensus of a "gold standard" method. Different methods have often been used interchangeably or cited as being comparable in the existing literature; however, the actual agreement between assays has not been investigated. Therefore..

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