Determination of angiotensin I-converting enzyme activity in equine blood: lack of agreement between methods of analysis
Maria Fernanda de M Costa, Adriana K Carmona, Marcio FM Alves, Timothy M Ryan, Helen M Davies, Garry A Anderson, Ron F Slocombe
Journal of Veterinary Science | KOREAN SOC VETERINARY SCIENCE | Published : 2011
Angiotensin-I converting enzyme (ACE) is a key regulator of blood pressure, electrolytes and fluid homeostasis through conversion of angiotensin I into angiotensin II. Recently, a genetic polymorphism of the ACE gene, which accounts for 47% of the variation of ACE activity in blood, has been advocated as a biomarker of athletic aptitude. Different methods of analysis and determination of ACE activity in plasma have been used in human and equine research without a consensus of a "gold standard" method. Different methods have often been used interchangeably or cited as being comparable in the existing literature; however, the actual agreement between assays has not been investigated. Therefore..View full abstract
This research was funded by a Joint Collaborative Grant from The University of Melbourne (Australia) and UNIFESP (Brazil), and by The Pathology Section of The Faculty of Veterinary Science at The University of Melbourne.