Journal article
Translocation of a bak C-Terminus mutant from cytosol to mitochondria to mediate cytochrome c release: Implications for bak and bax apoptotic function
PE Ferrer, P Frederick, JM Gulbis, G Dewson, RM Kluck
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2012
Abstract
Background: One of two proapoptotic Bcl-2 proteins, Bak or Bax, is required to permeabilize the mitochondrial outer membrane during apoptosis. While Bax is mostly cytosolic and translocates to mitochondria following an apoptotic stimulus, Bak is constitutively integrated within the outer membrane. Membrane anchorage occurs via a C-terminal transmembrane domain that has been studied in Bax but not in Bak, therefore what governs their distinct subcellular distribution is uncertain. In addition, whether the distinct subcellular distributions of Bak and Bax contributes to their differential regulation during apoptosis remains unclear. Methodology/Principal Findings: To gain insight into Bak and ..
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Awarded by AICR
Awarded by National Health and Medical Research Council
Funding Acknowledgements
This work was supported by grant #10-230 from the AICR http://www.aicr.org.uk and grants #637335 and #637337 from the National Health and Medical Research Council www.nhmrc.gov.au, as well as Future Fellowships (GD and RK) from the Australian Research Council http://www.arc.gov.au, and was made possible through Victorian State Government Operational Infrastructure Support and Australian Government National Health and Medical Research Council Independent Research Institute Infrastructure Support Scheme. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.