Journal article

Cutting edge: Generation of splenic CD8( ) and CD8(-) dendritic cell equivalents in fms-like tyrosine kinase 3 ligand bone marrow cultures

SH Naik, AI Proietto, NS Wilson, A Dakic, P Schnorrer, M Fuchsberger, MH Lahoud, M O'Keeffe, QX Shao, WF Chen, JA Villadangos, K Shortman, L Wu

JOURNAL OF IMMUNOLOGY | AMER ASSOC IMMUNOLOGISTS | Published : 2005

Abstract

We demonstrate that functional and phenotypic equivalents of mouse splenic CD8(+) and CD8(-) conventional dendritic cell (cDC) subsets can be generated in vitro when bone marrow is cultured with fms-like tyrosine kinase 3 (flt3) ligand. In addition to CD45RA(high) plasmacytoid DC, two distinct CD24(high) and CD11b(high) cDC subsets were present, and these subsets showed equivalent properties to splenic CD8(+) and CD8(-) cDC, respectively, in the following: 1) surface expression of CD11b, CD24, and signal regulatory protein-alpha; 2) developmental dependence on, and mRNA expression of, IFN regulatory factor-8; 3) mRNA expression of TLRs and chemokine receptors; 4) production of IL-12 p40/70, ..

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