Journal article
Defining the Antigenic Diversity of Plasmodium falciparum Apical Membrane Antigen 1 and the Requirements for a Multi-Allele Vaccine against Malaria
DR Drew, AN Hodder, DW Wilson, M Foley, I Mueller, PM Siba, AE Dent, AF Cowman, JG Beeson
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2012
Abstract
Apical Membrane Antigen 1 (AMA1) is a leading malaria vaccine candidate and a target of naturally-acquired human immunity. Plasmodium falciparum AMA1 is polymorphic and in vaccine trials it induces strain-specific protection. This antigenic diversity is a major roadblock to development of AMA1 as a malaria vaccine and understanding how to overcome it is essential. To assess how AMA1 antigenic diversity limits cross-strain growth inhibition, we assembled a panel of 18 different P. falciparum isolates which are broadly representative of global AMA1 sequence diversity. Antibodies raised against four well studied AMA1 alleles (W2Mef, 3D7, HB3 and FVO) were tested for growth inhibition of the 18 ..
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Funding Acknowledgements
Funding was provided by the PATH Malaria Vaccine Initiative; US Agency for International Development; National Health and Medical Research Council of Australia (program grant to JGB and AFC; Infrastructure for Research Institutes Support Scheme Grant); Australian Research Council (Future Fellowship to JGB); and a Victorian State Government Operational Infrastructure Support grant. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.