Journal article

IMMUNODETECTION METHODS FOR GRASS-POLLEN ALLERGENS ON WESTERN BLOTS

EK ONG, C SUPHIOGLU, MB SINGH, RB KNOX

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY | KARGER | Published : 1990

Abstract

A comparison is made of eight different methods to detect allergenic proteins in Western blots of rye-grass pollen extracts. Horseradish peroxidase-based enhanced chemiluminescence (ECL) provides a sensitive method for the detection of allergenic proteins. The method has been modified to use more dilute solutions of ECL substrate to reduce the background, can be applied to a standard nitrocellulose membrane, and used with Kodak X-ray film. The assays can be performed rapidly, replacing use of radiolabelled probes. Increased resolution is obtained. This makes the method suitable for detection of cDNA clones on plaque lifts, and for rapid and specific purification of proteins following immunod..

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University of Melbourne Researchers