The Stability and Complexity of Antibody Responses to the Major Surface Antigen of Plasmodium falciparum Are Associated with Age in a Malaria Endemic Area
Alyssa E Barry, Angela Trieu, Freya JI Fowkes, Jozelyn Pablo, Mina Kalantari-Dehaghi, Algis Jasinskas, Xiaolin Tan, Matthew A Kayala, Livingstone Tavul, Peter M Siba, Karen P Day, Pierre Baldi, Philip L Felgner, Denise L Doolan
Molecular and Cellular Proteomics | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2011
Individuals that are exposed to malaria eventually develop immunity to the disease with one possible mechanism being the gradual acquisition of antibodies to the range of parasite variant surface antigens in their local area. Major antibody targets include the large and highly polymorphic Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) family of proteins. Here, we use a protein microarray containing 123 recombinant PfEMP1-DBLα domains (VAR) from Papua New Guinea to seroprofile 38 nonimmune children (<4 years) and 29 hyperimmune adults (≥15 years) from the same local area. The overall magnitude, prevalence and breadth of antibody response to VAR was limited at 20) with age, cons..View full abstract
Awarded by National Health and Medical Research Council of Australia (NHMRC)
Awarded by National Institute of Allergy and Infectious Diseases
Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
We gratefully acknowledge the support of the PNG community and staff of the Papua New Guinea Institute of Medical Research. We would also like to acknowledge H. Imrie and J. Hume for assistance with sample collection, P. Michon for anti-VSA data and J. Beeson and J. Reeder for critical reading of the manuscript.This work was supported by the National Health and Medical Research Council of Australia (NHMRC, Project Grants 496600 and 1005653; http://www.nhmrc.gov.au) and the National Institute of Allergy and Infectious Diseases (Grant R43AI066791-01; http://www.nih.gov.au). Samples were collected with funding from a Wellcome Trust Project Grant awarded to KPD. AEB was supported by an Innovation Fellowship from the Victorian Endowment for Science Knowledge and Innovation and a Howard Florey Centenary Fellowship from the NHMRC. FJIF is supported by a training fellowship from the NHMRC. DLD is supported by a Pfizer Australia Senior Research Fellowship. We gratefully acknowledge the contribution of the Victorian Operational Infrastructure Support Program, through the Burnet Institute.