Targeting of Neisserial PorB to the mitochondrial outer membrane: an insight on the evolution of beta-barrel protein assembly machines
Jhih-Hang Jiang, John K Davies, Trevor Lithgow, Richard A Strugnell, Kipros Gabriel
Molecular Microbiology | WILEY-BLACKWELL | Published : 2011
Mitochondria originated from Gram-negative bacteria through endosymbiosis. In modern day mitochondria, the Sorting and Assembly Machinery (SAM) is responsible for eukaryotic β-barrel protein assembly in the mitochondrial outer membrane. The SAM is the functional equivalent of the β-barrel assembly machinery found in the outer membrane of Gram-negative bacteria. In this study we examined the import pathway of a pathogenic bacterial protein, PorB, which is targeted from pathogenic Neisseria to the host mitochondria. We have developed a new method for measurement of PorB assembly into mitochondria that relies on the mobility shift exhibited by bacterial β-barrel proteins once folded and separat..View full abstract
We thank Janette Tong, Allen Foo, Shauna Lyons-Schindler, Dr Catherine Ryan, Dr Sally Turner, Kher Shing Tan and Chenai Khoo for technical assistance and/or supply of plasmids and genomic DNA, Prof. Kostas Tokatlidis for the Tim10 depletions strain, Dr Jacqueline Gulbis for the anti-Tim10 antibody, Professor Nikolaus Pfanner for the anti-Sam50 antibody, Dr Ana Traven and Dr Felicity Alcock for comments on the manuscript. J.H.J. was supported by an Endeavour International Postgraduate Research Scholarship and a Melbourne International Research Scholarship from the University of Melbourne. T. L. is an Australian Research Council Federation Fellow. K. G. is funded by Monash University, the Eirene Loucas Foundation and the National Health and Medical Research Council of Australia.