Journal article
First molecular characterization of Giardia duodenalis from goats in Malaysia
YAL Lim, MAK Mahdy, TK Tan, XT Goh, AR Jex, MJ Nolan, RSK Sharma, RB Gasser
Molecular and Cellular Probes | Published : 2013
Abstract
In the present study, 310 faecal samples from goats from eight different farms in Malaysia were tested for the presence of Giardia using a PCR-coupled approach. The nested PCR for SSU amplified products of the expected size (∼200 bp) from 21 of 310 (6.8%) samples. Sixteen of these 21 products could be sequenced successfully and represented six distinct sequence types. Phylogenetic analysis of the SSU sequence data using Bayesian Inference (BI) identified Giardia assemblages A, B and E. The identification of the 'zoonotic' assemblages A and B suggests that Giardia-infected goats represent a possible reservoir for human giardiasis in Malaysia. © 2012 Elsevier Ltd.
Grants
Awarded by Australian Research Council
Funding Acknowledgements
The authors thank the farmers for their collaborative efforts and Bakhtiar Affendi Bukari for his technical assistance. We acknowledge the funding support from the Ministry of Higher Education, Malaysia, University of Malaya [projects RG221-10HTM (YLAL and RBG) and PV024/2011B (YLAL and TKT)]. Funding support from the Australian Research Council (ARC) and Melbourne Water Corporation are gratefully acknowledged (RBG and ARJ).