Journal article
RECK in osteosarcoma: A novel role in tumour vasculature and inhibition of tumorigenesis in an orthotopic model
JCM Clark, T Akiyama, DM Thomas, A Labrinidis, A Evdokiou, SJ Galloway, HS Kim, CR Dass, PFM Choong
Cancer | WILEY | Published : 2011
DOI: 10.1002/cncr.25757
Abstract
BACKGROUND: Targeted therapy in osteosarcoma (OS) is needed to improve patient outcomes. Human RECK may have a role because it inhibits cancer invasion and regulates angiogenesis. This study aimed to characterize RECK expression in human OS, to examine in vitro effects of RECK on vascular endothelium and OS cell behavior, and to analyze the effect of RECK on OS grown orthotopically in nude mice. METHODS: RECK was examined in human OS samples. Interactions between RECK and VEGF were studied in tissue and cells. RECK transfection was used to study its effects on vascular endothelial (HMEC-1) and OS (SaOS-2) cell behavior in vitro and in vivo. SaOS-2 co-culture with RAW 246.7-derived osteoclast..
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Funding Acknowledgements
This study was supported by a grant from the Australian Orthopaedic Association. Jonathan C. M. Clark was supported by scholarships from the Royal Australasian College of Surgeons, St Vincent's Hospital, and the National Health and Medical Research Council of Australia.