Spatiotemporal alterations of central α1-adrenergic receptor binding sites following amygdaloid kindling seizures in the rat: autoradiographic studies using [3H]prazosin

Abstract

Noradrenergic neurons are thought to be involved in the process of seizure development and long-term central nervous system plasticity associated with kindling and epilepsy. These processes involve actions of noradrenaline at α1-, α2- and β1-adrenergic receptors. In this study, quantitative in vitro autoradiography was used to investigate possible changes in the density of brain α1-adrenergic receptors in a kindling model of epilepsy in the rat. Kindling was produced by daily unilateral stimulation of the amygdala. The α1A + α1B subtypes of adrenergic receptors were labelled with the α1-selective antagonist, [3H]prazosin and α1B receptors, detected in the presence of 10 nM WB4101 to selectiv..