Induced somatic sector analysis of cellulose synthase (CesA) promoter regions in woody stem tissues
Nicky M Creux, Gerd Bossinger, Alexander A Myburg, Antanas V Spokevicius
PLANTA | SPRINGER | Published : 2013
The increasing focus on plantation forestry as a renewable source of cellulosic biomass has emphasized the need for tools to study the unique biology of woody genera such as Eucalyptus, Populus and Pinus. The domestication of these woody crops is hampered by long generation times, and breeders are now looking to molecular approaches such as marker-assisted breeding and genetic modification to accelerate tree improvement. Much of what is known about genes involved in the growth and development of plants has come from studies of herbaceous models such as Arabidopsis and rice. However, transferring this information to woody plants often proves difficult, especially for genes expressed in woody ..View full abstract
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Awarded by Australian Research Council
We are grateful to Martin Ranik for providing the CesA-promoter::GUS constructs which made this study possible and Minique De Castro for aiding in the construction of the second CAMV35S-promoter::GUS construct. We also thank Julio Najera, Valerie Frassiant and Angelique Manuel for laboratory assistance. This work was supported through funding provided by Mondi and Sappi to the Forest Molecular Genetics (FMG) Programme, the Technology and Human Resources for Industry Programme (THRIP) and the National Research Foundation (NRF) of South Africa as well as a Linkage Grant from the Australian Research Council (LP0776563) to GB, AAM and AVS.