Journal article

Inheritance of chromosome 7 is associated with a drug-resistant phenotype in somatic cell hybrids

M deSilva, P Kantharidis, DM Wall, L Campbell, V Vrazas, G Nadalin, SJ Kaczmarczyk, XF Hu, JD Parkin, JR Zalcberg

British Journal of Cancer | STOCKTON PRESS | Published : 1996

Abstract

A major form of drug resistance in tumour cells known as classical multidrug resistance (MDR) is associated with the overexpression of the mdr1 gene product, the membrane protein P-glycoprotein (P-gp), which acts as an energy-dependent drug efflux pump. In this study the inheritance of P-gp expression was examined using hybrids formed after somatic cell fusion between a drug-sensitive human T-cell leukaemia cell line, CEM/CCRF, and a drug-resistant derivative, CEM/A7, which is characterized by a clonal chromosomal duplication dup(7)(q11.23q31.2). Fourteen hybrids, chosen at random, were analysed by reverse transcriptase-polymerase chain reaction (RT-PCR) and by binding studies involving the ..

View full abstract