The challenges of using a copper fluorescent sensor (CS1) to track intracellular distributions of copper in neuronal and glial cells
Katherine A Price, James L Hickey, Zhiguang Xiao, Anthony G Wedd, Simon A James, Jeffrey R Liddell, Peter J Crouch, Anthony R White, Paul S Donnelly
CHEMICAL SCIENCE | ROYAL SOC CHEMISTRY | Published : 2012
This work was supported by funding from the National Health and Medical Research Council of Australia, the Australian Research Council and funds from the CSIRO OCE Postdoctoral Fellowship Program (SAJ). Aspects of this research were undertaken on the X-ray Fluorescence Microscopy beamline at the Australian Synchrotron. We would like to thank Prof. James Camakaris, Department of Genetics, The University of Melbourne for his valuable suggestions and input into this work. We thank Assoc. Prof. Andrew Hill and Dr Percy Chu, Department of Biochemistry and Molecular Biology, and Bio21 Institute, The University of Melbourne for help with live cell imaging. We also thank Dr Sharon La Fontaine, Dr Roxana Llanos and Mr Kenny Tran of Deakin University, Victoria, Australia for assistance with AAS, and Jens Brose and Lee Xin Chong of the University of Melbourne for isolation of Atox1 and CopK proteins.