Conference Proceedings

PARATHYROID HORMONE-RELATED PROTEIN - ISOLATION, MOLECULAR-CLONING, AND MECHANISM OF ACTION

TJ MARTIN, EH ALLAN, IW CAPLE, AD CARE, JA DANKS, H DIEFENBACHJAGGER, PR EBELING, MT GILLESPIE, G HAMMONDS, JA HEATH, PJ HUDSON, BE KEMP, M KUBOTA, SC KUKREJA, JM MOSELEY, KW NG, LG RAISZ, CP RODDA, HA SIMMONS, LJ SUVA Show all

RECENT PROGRESS IN HORMONE RESEARCH, VOL 45 | ACADEMIC PRESS INC | Published : 1989

Abstract

Many factors, such as interleukin 1, TGF alpha, tumor necrosis factor alpha and beta, and PGs, have been implicated in etiological roles in HHM (Martin and Mundy, 1987). Much interest in the past has also centered upon the likelihood of ectopic secretion of PTH in this condition. We have purified a protein (PTHrP) implicated in HHM from a human lung cancer cell line (BEN). Full-length cDNA clones have been isolated and were found to encode a prepropeptide of 36 amino acids and a mature protein of 141 amino acids. Eight of the first 13 amino acids were identical with human PTH, although antisera directed to the NH2 terminus of PTHrP do not recognize PTH; this homology is not maintained in the..

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