Journal article

Changes in the chondrocyte and extracellular matrix proteome during post-natal mouse cartilage development

R Wilson, EL Norris, B Brachvogel, C Angelucci, S Zivkovic, L Gordon, BC Bernardo, J Stermann, K Sekiguchi, JJ Gorman, JF Bateman

Molecular and Cellular Proteomics | Published : 2012

Abstract

Skeletal growth by endochondral ossification involves tightly coordinated chondrocyte differentiation that creates reserve, proliferating, prehypertrophic, and hypertrophic cartilage zones in the growth plate. Many human skeletal disorders result from mutations in cartilage extracellular matrix (ECM) components that compromise both ECM architecture and chondrocyte function. Understanding normal cartilage development, composition, and structure is therefore vital to unravel these disease mechanisms. To study this intricate process in vivo by proteomics, we analyzed mouse femoral head cartilage at developmental stages enriched in either immature chondrocytes or maturing/hypertrophic chondrocyt..

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University of Melbourne Researchers

Grants

Awarded by National Health and Medical Research Council of Australia


Awarded by Deutsche Forschungsgemeinschaft


Funding Acknowledgements

This work was supported by National Health and Medical Research Council of Australia Grant 419237 and Deutsche Forschungsgemeinschaft Grants BR2304/5-1, BR2304/7-1, and SFB 829-B6. The project was also supported by the Victorian Government's Operational Infrastructure Support Program and was undertaken using infrastructure provided by the Australian Government through the National Collaborative Research Infrastructure Strategy and Bioplatforms Australia. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be