Journal article
Three-dimensional atlas of iron, copper, and zinc in the mouse cerebrum and brainstem
DJ Hare, JK Lee, AD Beavis, A Van Gramberg, J George, PA Adlard, DI Finkelstein, PA Doble
Analytical Chemistry | AMER CHEMICAL SOC | Published : 2012
DOI: 10.1021/ac300374x
Abstract
Atlases depicting molecular and functional features of the brain are becoming an integral part of modern neuroscience. In this study we used laser ablation-inductively coupled plasma-mass spectrometry (LA-ICPMS) to quantitatively measure iron (Fe), copper (Cu), and zinc (Zn) levels in a serially sectioned C57BL/6 mouse brain (cerebrum and brainstem). Forty-six sections were analyzed in a single experiment of approximately 158 h in duration. We constructed a 46-plate reference atlas by aligning quantified images of metal distribution with corresponding coronal sections from the Allen Mouse Brain Reference Atlas. The 46 plates were also used to construct three-dimensional models of Fe, Cu, and..
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Funding Acknowledgements
The development of the ISIDAS program by Grigory Dorokhov and Michael Lake from the Computational Research Centre of Expertise, UTS is gratefully acknowledged. D.H. would like to acknowledge the Australian Research Council for financial support through the Linkage Projects grant program.