High-Resolution Elemental Bioimaging of Ca, Mn, Fe, Co, Cu, and Zn Employing LA-ICP-MS and Hydrogen Reaction Gas
Jessica Lear, Dominic J Hare, Fred Fryer, Paul A Adlard, David I Finkelstein, Philip A Doble
ANALYTICAL CHEMISTRY | AMER CHEMICAL SOC | Published : 2012
Imaging of trace metal distribution in tissue sections by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) is typically performed using spatial resolutions of 30 μm(2) and above. Higher resolution imaging is desirable for many biological applications in order to approach the dimensions of a single cell. The limiting factor for increasing resolution is sensitivity, where signal-to-noise ratios are poor due to inherent background spectral interferences and reduced sample volume with decreasing laser beam diameter. Several prominent spectral interferences are present for a number of biologically relevant isotopes, including the (40)Ar(16)O(+) spectral interference on (56)..View full abstract
Awarded by ARC
The authors would like to graciously acknowledge the support of the Australian Research Council, Agilent Technologies, and Kennelec Scientific for providing funding for the research through the Linkage Grant Program of the ARC (LP100200254).