Journal article
Molecular networks involved in mouse cerebral corticogenesis and spatio-temporal regulation of Sox4 and Sox11 novel antisense transcripts revealed by transcriptome profiling
KH Ling, CA Hewitt, T Beissbarth, L Hyde, K Banerjee, PS Cheah, PZ Cannon, CN Hahn, PQ Thomas, GK Smyth, SS Tan, T Thomas, HS Scott
Genome Biology | Published : 2009
Open access
Abstract
Background: Development of the cerebral cortex requires highly specific spatio-temporal regulation of gene expression. It is proposed that transcriptome profiling of the cerebral cortex at various developmental time points or regions will reveal candidate genes and associated molecular pathways involved in cerebral corticogenesis.Results: Serial analysis of gene expression (SAGE) libraries were constructed from C57BL/6 mouse cerebral cortices of age embryonic day (E) 15.5, E17.5, postnatal day (P) 1.5 and 4 to 6 months. Hierarchical clustering analysis of 561 differentially expressed transcripts showed regionalized, stage-specific and co-regulated expression profiles. SAGE expression profile..
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Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
We thank Felix Ng, Sarah King and Caitlin Collins for technical assistance; Frank Weissenborn for assistance with tissue dissections; Ken Simpson and Keith Satterley for computational assistance. The P19 cell line was kindly provided by Dr K Jensen, School of Molecular and Biomedical Science, University of Adelaide, South Australia, Australia. This work was supported by a National Health and Medical Research Council fellowships (171601 and 461204 to HSS); National Health and Medical Research Council Grants 219176, 257501, 215201, and 257529 (to HSS, GKS, and S-ST), a fellowship from the Deutsche Forschungsgemeinschaft (to TB). PQT is a Pfizer Australia Research Fellow. K-HL was a recipient of the Melbourne International Fee Remission Scholarship (MIFRS) and Universiti Putra Malaysia Staff Training Scholarship (UPMSTS), and Adelaide International Fee Scholarship (AIFS) equivalent. The majority of DNA sequencing in production of SAGE libraries was performed by the Australian Genome Research Facility, which was established through the Commonwealth-funded Major National Research Facilities program.