Recognition of CD1d-sulfatide mediated by a type II natural killer T cell antigen receptor

Onisha Patel; Daniel G Pellicci; Stephanie Gras; Maria L Sandoval-Romero; Adam P Uldrich; Thierry Mallevaey; Andrew J Clarke; Jerome Le Nours; Alex Theodossis; Susanna L Cardell; Laurent Gapin; Dale I Godfrey; Jamie Rossjohn

Journal article

Recognition of CD1d-sulfatide mediated by a type II natural killer T cell antigen receptor

Onisha Patel, Daniel G Pellicci, Stephanie Gras, Maria L Sandoval-Romero, Adam P Uldrich, Thierry Mallevaey, Andrew J Clarke, Jerome Le Nours, Alex Theodossis, Susanna L Cardell, Laurent Gapin, Dale I Godfrey, Jamie Rossjohn

NATURE IMMUNOLOGY | NATURE PUBLISHING GROUP | Published : 2012

DOI: 10.1038/ni.2372

Abstract

Natural killer T cells (NKT cells) are divided into type I and type II subsets on the basis of differences in their T cell antigen receptor (TCR) repertoire and CD1d-antigen specificity. Although the mode by which type I NKT cell TCRs recognize CD1d-antigen has been established, how type II NKT cell TCRs engage CD1d-antigen is unknown. Here we provide a basis for how a type II NKT cell TCR, XV19, recognized CD1d-sulfatide. The XV19 TCR bound orthogonally above the A' pocket of CD1d, in contrast to the parallel docking of type I NKT cell TCRs over the F' pocket of CD1d. At the XV19 TCR-CD1d-sulfatide interface, the TCRα and TCRβ chains sat centrally on CD1d, where the malleable CDR3 loops dom..

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University of Melbourne Researchers

Dale Godfrey Author Microbiology and Immunology

Adam Uldrich Author Microbiology and Immunology

Daniel Pellicci Author Microbiology and Immunology

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IMMUNE REGULATION EFFECTOR FUNCTION AND THERAPY

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We seek to understand how white blood cells detect and destroy disease, and how molecules of the immune system punch holes in diseased cells..

RESEARCH FELLOWSHIP

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Self-reactivity by NKT cells

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Grants

Awarded by US National Institutes of Health

Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES

Funding Acknowledgements

We thank the staff at the MX2 beamline of the Australian synchrotron for assistance with data collection and Monash Macromolecular Crystallisation Facility for crystallization experiments. Supported by the National Health and Medical Research Council of Australia (D.I.G. and J.R.), the Australian Research Council, the Cancer Council of Victoria, the US National Institutes of Health (AI090450 and AI092108 to L.G.), the Swedish Research Council (S.L.C.), the Swedish Cancer Society (S.L.C.) and Monash University (S.C. and J.R.).