Distinguishing Hyperglycemic Changes by Set7 in Vascular Endothelial Cells
Jun Okabe, Christian Orlowski, Aneta Balcerczyk, Chris Tikellis, Merlin C Thomas, Mark E Cooper, Assam El-Osta
CIRCULATION RESEARCH | LIPPINCOTT WILLIAMS & WILKINS | Published : 2012
RATIONALE: Epigenetic changes are implicated in the persisting vascular effects of hyperglycemia. The precise mechanism whereby chromatin structure and subsequent gene expression are regulated by glucose in vascular endothelial cells remain to be fully defined. OBJECTIVE: We have studied the molecular and functional mechanism whereby the Set7 methyltransferase associates with chromatin formation and histone methylation in vascular cells in response to current and previous exposure to glucose. METHODS AND RESULTS: To characterize the molecular and functional identity of the Set7 protein, we used vascular cells overexpressing or lacking Set7. Chromatin fractionation for mono-methylation of lys..View full abstract
The authors acknowledge grant and fellowship support from the Juvenile Diabetes Research Foundation International (JDRF), the Diabetes Australia Research Trust (DART), the National Health and Medical Research Council (NHMRC), and the National Heart Foundation of Australia (NHF). A.E.-O. is a Senior Research Fellow supported by the NHMRC and M. E. C. is a NHMRC Australia Fellow and a recipient of a JDRF Scholars Award. This study was supported in part by the Victorian Government's Operational Infrastructure Support Program.