Journal article
Detection of Bcl-2 family member Bcl-G in mouse tissues using new monoclonal antibodies
M Giam, JD Mintern, GJP Rautureau, MG Hinds, A Strasser, P Bouillet
Cell Death and Disease | NATURE PUBLISHING GROUP | Published : 2012
Abstract
Bcl-G is an evolutionarily conserved member of the Bcl-2 family of proteins that has been implicated in regulating apoptosis and cancer. We have generated monoclonal antibodies that specifically recognise mouse Bcl-G and have used these reagents to analyse its tissue distribution and subcellular localisation using western blotting, immunohistochemistry and immunofluor-escence. We found that Bcl-G predominantly resides in the cytoplasm and is present in a wide range of mouse tissues, including the spleen, thymus, lung, intestine and testis. Immunohistochemical analyses revealed that Bcl-G is expressed highly in mature spermatids in the testis, CD8+ conventional dendritic cells (DCs) in hemato..
View full abstractGrants
Awarded by Leukaemia and Lymphoma Research
Funding Acknowledgements
We thank M Robati for technical assistance, LA O'Reilly for sharing reagents and expertise, G Siciliano, J Coughlin, E Sutherland and S O'Connor for mouse care, B Helbert and C Young for mouse genotyping, K Wycherley for mAb production, E Tsui, S Mihajlovic and their team for histological preparations. This work was supported by the Australian NHMRC (programme grant 461221, Independent Research Institutes Infrastructure Support Scheme grant 361646 and Career Development Award), the Leukaemia and Lymphoma Society (Specialized Centre of Research Grant 7015), and infrastructure support from the NHMRC (IRISS) and the Victorian State Government (OIS). M Giam was funded by the University of Melbourne under the Endeavour IPRS scholarship programme. JD Mintern is the recipient of an NHMRC Career Development Award.