Journal article

Comparative proteomic analysis of the venom of the taipan snake, Oxyuranus scutellatus, from Papua New Guinea and Australia: Role of neurotoxic and procoagulant effects in venom toxicity

Maria Herrera, Julian Fernandez, Mariangela Vargas, Mauren Villalta, Alvaro Segura, Guillermo Leon, Yamileth Angulo, Owen Paiva, Teatulohi Matainaho, Simon D Jensen, Kenneth D Winkel, Juan J Calvete, David J Williams, Jose Maria Gutierrez



The venom proteomes of populations of the highly venomous taipan snake, Oxyuranus scutellatus, from Australia and Papua New Guinea (PNG), were characterized by reverse-phase HPLC fractionation, followed by analysis of chromatographic fractions by SDS-PAGE, N-terminal sequencing, MALDI-TOF mass fingerprinting, and collision-induced dissociation tandem mass spectrometry of tryptic peptides. Proteins belonging to the following seven protein families were identified in the two venoms: phospholipase A(2) (PLA(2)), Kunitz-type inhibitor, metalloproteinase (SVMP), three-finger toxin (3FTx), serine proteinase, cysteine-rich secretory proteins (CRISP), and coagulation factor V-like protein. In additi..

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Awarded by Vicerrectoria de Investigacion (Universidad de Costa Rica)

Awarded by CRUSA-CSIC

Awarded by Ministerio de Ciencia e Innovacion, Madrid, Spain

Awarded by Generalitat Valenciana, Valencia, Spain

Funding Acknowledgements

The authors thank Dr Andres Hernandez and B.Sc. Julissa Fonseca (Instituto Clodomiro Picado) for their collaboration, as well as Dr Bruno Lomonte for performing some of the mass spectrometry analyses. This study was supported by CONARE, Vicerrectoria de Investigacion (Universidad de Costa Rica) (projects 741-A7-611 and 741-A9-506), CRUSA-CSIC (Project 2009CR0021), Ministerio de Ciencia e Innovacion, Madrid, Spain (grant BFU2010-17373), Generalitat Valenciana, Valencia, Spain (grant PROMETEO/2010/005), the PNG Office of Higher Education, CTP Limited (Milne Bay Estates), and the Australian Venom Research Unit (University of Melbourne), which is funded by the Australian Government Department of Health and Ageing, the Australia Pacific Science Foundation and Snowy Nominees. Analyses performed at the Proteomics Laboratory of Instituto Clodomiro Picado were supported by CONARE and Vicerrectoria de Investigacion, Universidad de Costa Rica.