Journal article

DESCRIPTION OF A MOUSE MONOCLONAL ANTI-HLA-B27 ANTIBODY HLA-ABC-M3

JA TRAPANI, HA VAUGHAN, RL SPARROW, BD TAIT, IFC MCKENZIE

HUMAN IMMUNOLOGY | ELSEVIER SCIENCE INC | Published : 1983

Abstract

The production and characterization of a new anti-HLA-B27 monoclonal antibody HLA-ABC-m3 is described. This cytotoxic IgG2a antibody binds protein A and is able to precipitate cell surface molecules of 43,000 and 12,000 daltons corresponding to the HLA heavy chain and beta 2-microglobulin. Population testing revealed that the HLA-ABC-m3 antibody reacted with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27+ and with 5/105 HLA-B27- individuals. These five extra reactions were with individuals expressing the cross-reactive HLA-B7 alloantigen, although the affinity of the monoclonal antibody for B27 heterozygous individuals (approx 10(9) M-1) was tenfold gre..

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