Microwave Synthesis of Prion Protein Fragments up to 111 Amino Acids in Length Generates Biologically Active Peptides
John A Karas, Martin Boland, Cathryn Haigh, Vanessa Johanssen, Andrew Hill, Kevin Barnham, Steven Collins, Denis Scanlon
International Journal of Peptide Research and Therapeutics | Springer Verlag | Published : 2012
Misfolded conformers of the prion protein are aetiologically implicated in neurodegenerative conditions termed prion diseases (also known as transmissible spongiform encephalopathies). Two constitutively expressed N-terminal peptides corresponding to human residues 23–90 and 23–111 are thought to serve normal physiological roles related to neuronal protection with membrane binding possibly playing a part in their mechanism of action. These peptides, along with several derivatives up to 111 residues in length, have been produced by microwave assisted peptide synthesis. HPLC and MS characterisation showed that the peptides were manufactured in good yields at high purity. Peptides were assayed ..View full abstract
Awarded by NHMRC
We thank Dr Sen Han for the use of the schematic PrP protein structure shown in Fig. 1. SC is supported by an NH&MRC Practitioner Fellowship #APP1005816 and Program Grant #628946.