Isolation, expansion and characterisation of alloreactive human Th17 and Th1 cells
K Kerry Ko, Maree S Powell, Eva Orlowski, Sara Prickett, Doreen Krumbiegel, P Mark Hogarth
IMMUNOLOGY LETTERS | ELSEVIER SCIENCE BV | Published : 2012
Interleukin 17 producing T helper cells (Th17) and IFNγ producing Th1 cells are distinct subsets of effector memory CD4(+) T cells that are crucial to host immunity and have been linked to the pathology of certain inflammatory autoimmune diseases. We have developed a method for the isolation and long term culture of human Th17 and Th1 cells. Using allogeneic stimulation we have cultured homogeneous populations of Th17 and Th1 cells to large cell numbers. These alloreactive cell lines were established from CD4(+)CD45RO(+) memory T cells expressing, or lacking, CCR6 and CCR4. The Th17 cells were derived only from cells expressing both CCR6 and CCR4 whereas the Th1 cells, secreting IFNγ, were d..View full abstract
This work was funded by National Health and Research Council Project grants and the Collaborative Research Centre for Biomarker Translation and the Victorian Operational Infrastructure Scheme. KK is supported by Arthritis Australia. PMH is an NHMRC SPRF. The authors also wish to thank Bruce D. Wines and Janine Stubbs for helpful discussions and comment on the manuscript.