Journal article
Expression of pro- and antiapoptotic molecules of the Bcl-2 family in human islets postisolation
PD Campbell, A Weinberg, J Chee, L Mariana, R Ayala, WJ Hawthorne, PJ O'Connell, T Loudovaris, MJ Cowley, TW Kay, ST Grey, HE Thomas
Cell Transplantation | Published : 2012
Abstract
Human islets are subjected to a number of stresses before and during their isolation that may influence their survival and engraftment after transplantation. Apoptosis is likely to be activated in response to these stresses. Apoptosis due to intrinsic stresses is regulated by pro- and antiapoptotic members of the Bcl-2 family. While the role of the Bcl-2 family in apoptosis of rodent islets is becoming increasingly understood, little is known about which of these molecules are expressed or required for apoptosis of human islets. This study investigated the expression of the Bcl-2 family of molecules in isolated human islets. RNA and protein lysates were extracted from human islets immediatel..
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Funding Acknowledgements
This study was supported by The Australian Federal Department of Health and Ageing and the Juvenile Diabetes Research Foundation International. We thank the Tom Mandel Islet Transplant Program and The Centre for Transplant and Renal Research, Westmead Millennium Institute for Medical Research for isolated human islets, Stacey Fynch and Gaurang Jhala for excellent technical assistance, and Professor Andreas Strasser and Dr. Lorraine O'Reilly from the Walter and Eliza Hall Institute of Medical Research for antibodies. We thank Dr. Helen Speirs from the Ramaciotti Centre for technical assistance and advice regarding microarrays. The authors declare no conflicts of interest.