Journal article
Characterization of thrombopoietin (TPO)-responsive progenitor cells in adult mouse bone marrow with in vivo megakaryocyte and erythroid potential
AP Ng, M Kauppi, D Metcalf, LD Rago, CD Hyland, WS Alexander
Proceedings of the National Academy of Sciences of the United States of America | Published : 2012
Abstract
Hematopoietic progenitor cells are the progeny of hematopoietic stem cells that coordinate the production of precise numbers of mature blood cells of diverse functional lineages. Identification of cell-surface antigen expression associated with hematopoietic lineage restriction has allowed prospective isolation of progenitor cells with defined hematopoietic potential. To clarify further the cellular origins of megakaryocyte commitment, we assessed the in vitro and in vivo megakaryocyte and platelet potential of defined progenitor populations in the adult mouse bone marrow. We show that megakaryocytes arise from CD150+ bipotential progenitors that display both platelet- and erythrocyte-produc..
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Awarded by Australian National Health and Medical Research Council
Awarded by Independent Research Institutes Infrastructure Support Scheme
Funding Acknowledgements
We thank Stephen Nutt and Sebastian Carotta for their generous gift of PU.1<SUP>gfp</SUP> mice; Lauren Wilkins, Merle Dayton, Kelly Trueman, and Jaclyn Gilbert for excellent animal husbandry; and Jason Corbin and Janelle Lochland for excellent technical assistance. This work was supported by Australian National Health and Medical Research Council Program Grant 461219, by Independent Research Institutes Infrastructure Support Scheme Grant 361646, and by fellowships from the Australian National Health and Medical Research Council (to W.S.A. and A.P.N.); the Carden Fellowship Fund of the Cancer Council, Victoria (to D.M.); a Haematology Society of Australia New Zealand/AMGEN New Investigator Scholarship (to A.P.N.); a Cure Cancer Australia/Leukaemia Foundation postdoctoral fellowship (to A.P.N.); the Australian Cancer Research Fund; and a grant from the Victorian State Government Operational Infrastructure Support.