Journal article

Two distinct types of murine blast colony-forming cells are multipotential hematopoietic precursors

D Metcalf, KT Greig, CA de Graaf, SJ Loughran, WS Alexander, M Kauppi, CD Hyland, L Di Rago, S Mifsud

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | NATL ACAD SCIENCES | Published : 2008

Abstract

Two types of blast colonies can be stimulated to develop in semisolid agar cultures of murine bone marrow cells. Typically, these are either multicentric colonies stimulated by stem cell factor (SCF) plus interleukin-6 (IL-6) or dispersed colonies stimulated by Flt3 ligand (FL) plus IL-6. Both types of blast colony-forming cells (BL-CFCs) can generate large numbers of lineage-committed granulocyte-macrophage progenitor cells and exhibit some capacity for self-generation and the formation of eosinophil and megakaryocyte progenitor cells. However, the two populations of BL-CFCs are largely distinct and partially separable by fluorescence-activated cell sorting and are distinguished by differin..

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Funding Acknowledgements

This work was supported by the Carden Fellowship Fund of the Cancer Council, Victoria, the National Health and Medical Research Council, Grant 461219, and National Institutes of Health Grant CA22556. C.A.d.G. and S.J.L. are recipients of Australian Postgraduate Awards, and K.T.G. is the recipient of a Stella Mary Langford Scholarship of the University of Melbourne.