Conference Proceedings

Use of thiazolidine-mediated ligation for site specific biotinylation of mouse EGF for biosensor immobilisation

JD Wade, T Domagala, J Rothacker, B Catimel, E Nice

LETTERS IN PEPTIDE SCIENCE | SPRINGER | Published : 2001

Abstract

Biosensors provide a sophisticated and discriminating means of probing biomolecular interactions. Specific ligands such as peptides and proteins can be immobilized onto sensor surfaces by a number of means including covalent attachment via amine, thiol or aldehyde chemistry, capture via biotin-avidin interaction or the use of specific tags. We have devised a simple chemoselective ligation method to selectively conjugate an anchoring functionality onto N-terminal serine or threonine residues of peptides and proteins allowing them to be immobilised onto the sensor surface in a defined orientation. It is based on the specific reaction of the 1,2-aminothiol of cysteine with an aldehyde under aci..

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