Journal article

Inhibition of HIV-1 virus replication using small soluble Tat peptides

E Agbottah, NG Zhang, S Dadgar, A Pumfery, JD Wade, Z Chen, F Kashanchi

VIROLOGY | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2006

Abstract

Although the introduction of highly active antiretroviral therapy (HAART) has led to a significant reduction in AIDS-related morbidity and mortality, unfortunately, many patients discontinue their initial HAART regimen, resulting in development of viral resistance. During HIV infection, the viral activator Tat is needed for viral progeny formation, and the basic and core domains of Tat are the most conserved parts of the protein. Here, we show that a Tat 41/44 peptide from the core domain can inhibit HIV-1 gene expression and replication. The peptides are not toxic to cells and target the Cdk2/Cyclin E complex, inhibiting the phosphorylation of serine 5 of RNAPII. Using the Cdk2 X-ray crysta..

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