Growth restriction before and after birth increases kinase signaling pathways in the adult rat heart
GD Wadley, ME Wlodek, G Ng, C Goodman, C Stathis, GK McConell
JOURNAL OF DEVELOPMENTAL ORIGINS OF HEALTH AND DISEASE | CAMBRIDGE UNIV PRESS | Published : 2010
To investigate the mechanisms for the previously reported development of adult cardiac hypertrophy in male rats following growth restriction, the levels of oxidative stress and activation of signaling kinases were measured in the left ventricle (LV) of adult rat offspring. In experiment one, bilateral uterine vessel ligation to induce uteroplacental insufficiency and growth restriction in the offspring (Restricted) or sham surgery was performed during pregnancy. Litters from sham mothers had litter size either reduced (Reduced Litter), which also restricted postnatal growth, or were left unaltered (Control). In males, Reduced Litter offspring had increased LV phosphorylation of AMPKα, p38 MA..View full abstract
Awarded by Heart Foundation of Australia
The authors would like to thank Kerryn Westcott and Dr Andrew Siebel for their technical assistance. All authors have contributed substantially to the scientific process leading up to the writing of the paper. GW, MW and GM contributed to the conception and design of the project. All authors contributed to the performance of experiments or the analysis and interpretation of data. All authors made a contribution to drafting of the manuscript. We the authors declare that we are entirely responsible for the scientific content of the paper. This work was supported by a grant (no. G06M 2597) from the Heart Foundation of Australia.