Journal article
HIV-1 infection of human macrophages directly induces viperin which inhibits viral production
N Nasr, S Maddocks, SG Turville, AN Harman, N Woolger, KJ Helbig, J Wilkinson, CR Bye, TK Wright, D Rambukwelle, H Donaghy, MR Beard, AL Cunningham
Blood | Published : 2012
Abstract
Macrophages are key target cells for HIV-1. HIV-1BaL induced a subset of interferon-stimulated genes in monocyte-derived macrophages (MDMs), which differed from that in monocyte-derived dendritic cells and CD4 T cells, without inducing any interferons. Inhibition of type I interferon induction was mediated by HIV-1 inhibition of interferon-regulated factor (IRF3) nuclear translocation. In MDMs, viperin was the most up-regulated interferon-stimulated genes, and it significantly inhibited HIV-1 production. HIV-1 infection disrupted lipid rafts via viperin induction and redistributed viperin to CD81 compartments, the site of HIV-1 egress by budding in MDMs. Exogenous farnesol, which enhances me..
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Awarded by Australian National Health and Medical Research Council
Funding Acknowledgements
This work was supported by the Australian National Health and Medical Research Council (project grant 632638 and program grant 358399).